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Course_TUDortmund_DE
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Course_UGhent_EN
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Course_ZHAW_Bioprocessing
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Course_ZHAW_BPT_DE
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Exercise_B0232_DE
Welche Eingriffe in die Auslegung des Experiments beeinflussen die Dauer des Prozesses oder dessen Phasen?


Exercise_BCCA_002_DE
Wie schnell und nach welcher Kinetik wird das Produkt gebildet?


Exercise_BCCA_003_DE
Erlaubt die Struktur des metabolischen Netzwerkes die Berechnung aller Reaktionsraten?


Exercise_BCCA_004_DE
Wie wird die Syntheserate von Aceton bestimmt?


Exercise_BCNN_001_CZ
Jak změníte dobu trvání vsádkové kultivace?


Exercise_BCNN_001_DE
Wie verändern Sie die Dauer eines Batchversuches?


Exercise_BCNN_001_DE
Wie beeinflussen Sie die Dauer eines Batchversuches?


Exercise_BCNN_005_DE
Wie wirkt sich eine Veränderung von YX/S auf den Verlauf des Batchversuches aus?


Exercise_BCNN_006_DE
Welchen Einfluss hat µmax auf den Verlauf des Batchversuches?


Exercise_BCNN_007_DE
Welchen Einfluss übt KS auf den Verlauf von µ(t) aus?


Exercise_ESBS001
ESBS001_Welche Eingriffe in die Auslegung des Experiments beeinflussen die Dauer des Wachstumsversuchs?


Exercise_ESBS002
Welches Modell bildet die Messdaten aus Ihrem Batchversuch nach?


Exercise_ESBS003
ESBS003_Was ist der Gleichgewichtszustand (steady-state) in einer kontinuierlichen Kultur und wann wird dieser erreicht?


Exercise_ESBS004
ESBS004_Wie werden aus zeitlich abhängigen Chemostatdaten x(D) und s(D) Diagramme erstellt?


Exercise_ESBS005
ESBS005_Unter welchen Bedingungen ist 1. die Biomassproduktivität und 2. die Produktivität für ein Enzym am höchsten, dessen Expression durch den Methanoloxidase-Promotor kontrolliert wird?


Exercise_ESBS006
ESBS006_Wie verändert sich die spezifische Wachtumsrate im Verlauf des Batchwachstums?


Exercise_ESBS007
ESBS007_Welchen Einfluss haben Wachstumsparameter und sin auf x(D) und s(D)?


Exercise_ETH/Uni05
ETH/Uni05_With the help of which model can you describe the experimental data of Kayser et al. (2004)?


Exercise_ETH/Uni12
ETH/Uni12_Continuous culture: interpretation of experimental data.


 
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References



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Blank, L. M. 1997. Molecular and fermentation characterization of recombinant strains for metabolic engineering studies of Clostridium acetobutylicum. Master Thesis. Northwestern University, Evanston, IL, USA.
Cereghino, J.L., and J.M. Cregg. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24: 45-66.
483 kBpdf
Curvers, S., P. Brixius, T. Klauser, J. Thoemmes, B.D. Weuster, R. Takors, and C. Wandrey. 2001. Human chymotrypsinogen B production with Pichia pastoris by integrated development of fermentation and downstream processing. Part 1. Fermentation. Biotechnol. Prog. 17: 495-502.
213 kBpdf
d’Anjou, M.C., and A.J. Daugulis. 2001. A rational approach to improving productivity in recombinant Pichia pastoris fermentation. Biotechnol. Bioeng. 72: 1–11.
214 kBpdf
Desai, R.P., L.K. Nielsen, and E.T. Papoutsakis. 1999. Stoichiometric modeling of Clostridium acetobutylicum fermentations with non-linear constraints. J. Biotechnol. 71:191-205.
230 kBpdf
Egli, T., Bosshard, C., and G. Hamer. 1986. Simultaneous utilization of methanol-glucose mixtures by Hansenula polymorpha in chemostat: Influence of dilution rate and mixture composition on utilization pattern. Biotechnol. Bioneng. 28: 1734-1741.
903 kBpdf
Egli, T., J.P. van Dijken, M. Veenhuis, W. Harder, and A. Fiechter. 1980. Methanol Metabolism in Yeasts: Regulation of the Synthesis of Catabolic Enzymes. Arch. Microbiol. 124: 115-121.
1777 kBpdf
Hans-Peter Meyer, 7. Juni 2007
1307 kBpdf
Harris, L.M., L. Blank, R.P. Desai, N.E. Welker, and E.T. Papoutsakis. 2001. Fermentation characterization and flux analysis of recombinant strains of Clostridium acetobutylicum with an inactivated solR gene. J. Ind. Microbiol. Biotechnol. 27: 322-328.
167 kBpdf
Ihssen, J., and T. Egli. 2004. Specific growth rate and not cell density controls the general stress response in Escherichia coli. Microbiol. 150: 1637 – 1648.
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Invitrogen. 2002. Pichia Fermentation Process Guidelines. Catalog No. K1710-01, Version B 053002, 1-11. http://www.invitrogen.com/content/sfs/manuals/pichiaferm_prot.pdf
162 kBpdf
Kayser, A., J. Weber, V. Hecht, and U. Rinas. 2005. Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture. I. Growth-rate-dependent metabolic efficiency at steady state. Microbiol. 151: 693 - 706.
226 kBpdf
Kovářová, K., A.J.B. Zehnder, and T. Egli. 1996. Temperature-Dependent Growth Kinetics of Escherichia coli ML 30 in Glucose-Limited Continuous Culture. J. Bacteriol. 178: 4530-4539.
340 kBpdf
Kovarova-Kovar, K., and T. Egli. 1998. Growth Kinetics of Suspended Microbial cells: From Single-Substrate-Controlled Growth to Mixed-Substrate Kinetics. Microbiol. Mol. Biol. Rev. 62: 646–666.
2980 kBpdf
Lendenmann, U., H. Senn, M. Snozzi, and T. Egli. 2000. Dynamics of Mixed Substrate Growth of Escherichia coli in Batch Culture: the Transition between Simultaneous and Sequential Utilisation of Carbon Substrates. Acta Univ. Carol. Environ. 14: 21-31.
2509 kBpdf
Maurer, M., M., Kühleitner, B. Gasser, and D. Mattanovich. 2006. Versatile modeling adn optimization of fed  batch processes for the production of secreted heterologous proteins with Pichia pastoris. Microbial Cell Factories. 5: 37.
323 kBpdf
Minning, S., A. Serrano, P. Ferrer, C. Solá, R.D. Schmid, and F. Valero. 2001. Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J. Biotechnol. 86: 59–70.
196 kBpdf
Monod, J. 1949.The growth of bacterial cultures. Annu. Rev. Microbiol. 3: 371-394.
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Papoutsakis, E.T. 1984. Equations and Calculations for Fermentations of Butyric Acid Bacteria. Biotechnol. Bioeng. XXVI: 174-187.
1295 kBpdf
Piñar, G., K. Kovářová, T. Egli, and J.L. Ramos. 1998. Influence of Carbon Source on Nitrate Removal by Nitrate tolerant Klebsiella oxytoca CECT 4460 in Batch and Chemostat Cultures. Appl. Env. Microbiol. 64: 2970 - 2976. 
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Standing, C.N., A.G. Frederickson, and H.M. Tschuchiya. 1972. Batch and continuous-culture transients for two substrate systems. Appl. Microbiol. 23: 354-359.
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Tao, H., C. Bausch, C. Richmond, F.R. Blattner, and T. Conway. 1999. Functional Genomics: Expression Analysis of Escherichia coli. Growing on Minimal and Rich Media. J. Bacteriol. 181: 6425 - 6440.
1787 kBpdf
van Dijken, J.P., Otto, R., and W. Harder. 1976. Growth of Hansenula polymorpha in a methanol-limited chemostat. Arch. Microbiol. 111: 137-144.
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Varma, A., and B.O. Palsson. 1994. Stoichiometric Flux Balance Models Quantitatively Predict Growth and Metabolic By-Product Secretion in Wild-Type Escherichia coli W3110. Appl. Environ. Microbiol. 60: 3724-3731.
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von Meyenburg, K. 1971. Transport-Limited Growth Rates in a Mutant of Escherichia coli. J. Bacteriol. 107: 878-888.
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Wanner, U., and T. Egli. 1990. Dynamics of microbial growth and cell composition in batch culture. FEMS Microbiol. Rev. 75: 19-44.
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Zhang, W., J. Sinha, L. A. Smith, M. Inan, and M.M. Meagher. 2005. Maximization of production of secreted recombinant proteins in Pichia pastoris fed batch fermentation. Biotechnol. Prog. 21: 386-393.
195 kBpdf
 
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